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precipitates in 50X TAE

TAE buffer

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2 replies to this topic

#1 kshanik

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Posted 16 November 2011 - 08:02 AM

dear all,

i recently made 50X tae buffer and i notice some precipitates. is it normal??

many thanks.

S

#2 mole

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Posted 16 November 2011 - 08:15 AM

Hi Kshanik,

No, it should not.

It may be EDTA ppts.

If you have added EDTA as solution then just check your EDTA reagent bottle or its pH.

or if you have added solid then its again pH a problem.

I would recomend you to prepare fresh EDTA 0.5M pH 8.0, and prepare TAE freshly.

As its better to throw doubtful reagent than spoiling experiment.

Regards,

Mole.

#3 kshanik

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Posted 16 November 2011 - 11:17 PM

Dear Mole,

Thank you very much.





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