dear all,
i recently made 50X tae buffer and i notice some precipitates. is it normal??
many thanks.
S
0
2 replies to this topic
#2
mole
-
- Active Members
-
- 24 posts
member
0
Neutral
Neutral
Posted 16 November 2011 - 08:15 AM
Hi Kshanik,
No, it should not.
It may be EDTA ppts.
If you have added EDTA as solution then just check your EDTA reagent bottle or its pH.
or if you have added solid then its again pH a problem.
I would recomend you to prepare fresh EDTA 0.5M pH 8.0, and prepare TAE freshly.
As its better to throw doubtful reagent than spoiling experiment.
Regards,
Mole.
No, it should not.
It may be EDTA ppts.
If you have added EDTA as solution then just check your EDTA reagent bottle or its pH.
or if you have added solid then its again pH a problem.
I would recomend you to prepare fresh EDTA 0.5M pH 8.0, and prepare TAE freshly.
As its better to throw doubtful reagent than spoiling experiment.
Regards,
Mole.
#3
kshanik
-
- Active Members
-
- 5 posts
member
0
Neutral
Neutral
Posted 16 November 2011 - 11:17 PM
Dear Mole,
Thank you very much.
Thank you very much.
Also tagged with one or more of these keywords: TAE, buffer
 |
Protocols and Techniques Forums →
SDS-PAGE and Western Blotting →
5x SDS lysis bufferStarted by Guest_JoeSus_* , 31 May 2013  lysis, buffer, protein
|
|
|
|
Protocols and Techniques Forums →
Molecular Biology →
Agarose gel bands gone (for gel extraction)Started by Guest_relevant_username_* , 01 May 2013 DNA, agarose gel, bands, TAE and 1 more...
|
|
|
||
Protocols and Techniques Forums →
Chemistry →
EDTA precipitates out of EDTA-oxalate bufferStarted by Guest_Sandrako_* , 02 Apr 2013 buffer, flocculation, EDTA
|
|
|
||
Protocols and Techniques Forums →
General Lab Techniques →
|
|
|
||
Protocols and Techniques Forums →
SDS-PAGE and Western Blotting →
Blocking buffer for phospho-(AKT, GSK3b) WB using Odyssey?Started by Guest_proteinrunner_* , 25 Feb 2013 Odyssey, buffer, phospho
|
|
|
