Since native page never worked with my protein I started to run BN-gels as it is supposed to be a good tool for hydrophobic proteins. I'm using gradient gels (3-16 %) because the protein seems to form high aggregated complexes.
There is still one problem. I got protein smears so that I cannont perform Western blots. Attached you find an example. In the first two lines I loaded two different markers; first is BSA, second JackBean Urease. Then different protein samples follow. As you can see only BSA makers shows a nice distinct band.
I found several protocols and tried so far the one from Wittig et al (http://www.nature.co...ot.2006.62.html) and lately the one attached.
Since I have no real experience with native pages I would appreciate any suggestion to get rid of the smears.