sample storage method for extracting 16s rRNA
Posted 15 November 2011 - 12:38 PM
Are there any special needs? For instance, adding agents to stabilize DNA?
Thanks a lot!
Posted 15 November 2011 - 02:51 PM
Posted 15 November 2011 - 06:58 PM
Posted 25 March 2012 - 06:56 PM
1. which general procedures should I go through? I think it includes: DNA extraction-->get primers for the specific 16srRNA--> do common PCR to check if the primers are good-->do RT-PCR (include positive and negative control)-->data analysis. Is that correct?
2. The sample contains mammal cells and bacteria (gram- and grm+). I am interested in a G+ bacteria 16srRNA. As far as I know, the G+ bacteria DNA are harder to extract. How should I extract the DNA?
3. How should I design the primers for 16srRNA? Which database is good for designing the specific primer?
4. If the extracted DNA contains mammal cells DNA, and bacteria DNA, how should I quantify the DNA to make sure I load the same amount of bacteria DNA?
Thanks a lot! Look forward to your responses!