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RNA extraction from FFPE cancer tissues for real-time RT-PCR

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2 replies to this topic

#1 feifei83



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Posted 15 November 2011 - 05:29 AM

Hi, I need to extract RNA from FFPE cancer tissues for real-time RT-PCR. (The fresh frozen tissues are not available.) Does anyone have any recommendation of the extraction kit? Thank you.

#2 Trof


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Posted 18 November 2011 - 12:13 AM

If you'll hold for some time I will tell you, I'm currently solving the same problem. I should test two kits within few weeks and I will tell you.

I posted same question, but so far no responses - Real-time mRNA expression studies on FFPE samples

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#3 PostDocTrauma



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Posted 05 December 2011 - 03:31 AM

recoverall from ambion is what we use in the lab.

you just need to have enough of the ffpe material, i think we use 2x10micron sections. also cut away the old face as that will have many degraded nucleic acids

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