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how to form a pellet in pellet culture of MSC

pellet culture MSC

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#1 neverstall

neverstall

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Posted 15 November 2011 - 12:03 AM

I am now working on pellet culturing rat MSC with chondrogenic medium in 96well v-bottom plate. Normally, a pellet ball should form at day3. But what I got now are just tiny little cell colonies that sticked at the bottom of the well. I didn't centrifuge the plate after adding the cells. Is that step crucial in forming a pellet??

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