Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

PAGE gel recipes

acrylamide bisacrylamide

  • Please log in to reply
1 reply to this topic

#1 tigersgenome



  • Members
  • Pip
  • 4 posts

Posted 14 November 2011 - 06:27 PM

Hello, I am new to the forum...

Can anyone help me with a standard protocol for PAGE and SDS PAGE.

I need to prepare the recipes. But the different protocols, what I got from web sources, didnt work out.

Thank you.

especially the problem comes with dissolving of acrylamide and bis acrylamide.

#2 mdfenko


    an elder emeritus

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 3,265 posts

Posted 21 November 2011 - 08:01 AM

what is your problem with dissolving acrylamide and bis?

the solution gets cold, keep stirring until it is all dissolved (may take a while). i wouldn't heat the solution, just let it get to room temperature (all will be dissolved) on its own.

what other problems are you having with the protocols?
talent does what it can
genius does what it must
i do what i get paid to do

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.