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Beta-gal expression in Nucleus or cytoplasm

in vitro X-gal

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#1 Rnotk

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Posted 14 November 2011 - 01:50 PM

I have question about X-gal staining in in vitro culture.

I have primary culture from transgenic mouse with LacZ reporter.

When I did some treatments and stained with X-gal, I saw some cells become blue in its cytoplasm, but majority are blue in nucleus.

Is it normal??

Theoretically, I thought it should appear in cytoplasm.

so X-gal positive should appear in nucleus? if so does anyone know why?
and blue staining in cytoplasm means something different?

Any opinions, suggestions helps

Thanks in advance





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