Hi
I am currently helping one of our phd students with the development of a bio assay, we need to disaggregate some micro-algae into single cells but wish to keep the cells intact, plants are not an area of expertise for me, u have tried mechanical and ultrasonic disruption but these have had limited success.
Can anyone suggest a method for disaggregating the cells ?
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bob1
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Thelymitra pulchella
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Excellent
Excellent
Posted 14 November 2011 - 01:40 PM
Which methods of mechanical disruption did you use?
If you know what is linking them (cellulose?) you could perhaps use an enzyme digest.
If you know what is linking them (cellulose?) you could perhaps use an enzyme digest.
Edited by bob1, 14 November 2011 - 01:40 PM.
