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Disaggregation of micro-algae


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#1 ickypimp

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Posted 14 November 2011 - 08:57 AM

Hi

I am currently helping one of our phd students with the development of a bio assay, we need to disaggregate some micro-algae into single cells but wish to keep the cells intact, plants are not an area of expertise for me, u have tried mechanical and ultrasonic disruption but these have had limited success.

Can anyone suggest a method for disaggregating the cells ?

#2 bob1

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Posted 14 November 2011 - 01:40 PM

Which methods of mechanical disruption did you use?

If you know what is linking them (cellulose?) you could perhaps use an enzyme digest.

Edited by bob1, 14 November 2011 - 01:40 PM.





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