3 replies to this topic

[cancerlab]

Hi!

I do western blot regularly to check expression of protein X in 6 different cell lines. Now I've come across a problem with one cell line.  When I run w.b. with this cell line's new cell lysate, I see different  expression level (higher or lower) compared with my last result. I have no problem with the other 5 samples, but only this one.

What is the problem? Why this cell line's protein expression is not consistent with its last lysate?

p.s. I do not use any treatment. The origin of these cells are the same (colorectal). the passage and confluency is the same. the antibodies are the same, too.

Plz help me..................................

[EvilTwin]

Are these normal or cancer cells?

[cancerlab]

EvilTwin, on 03 January 2012 - 03:08 AM, said:

Are these normal or cancer cells?

Thanks for your reply. They aye all cancer cells.

[EvilTwin]

I think it might be the cause. Cancer cell are genetically  unstable and some of their features may change division-to-division.

Edited by EvilTwin, 09 January 2012 - 01:17 AM.