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Expression of protein X varies in different w.b running (in one cell line, but

protein western blot expression

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#1 cancerlab

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Posted 13 November 2011 - 08:36 PM

Hi!

I do western blot regularly to check expression of protein X in 6 different cell lines. Now I've come across a problem with one cell line. When I run w.b. with this cell line's new cell lysate, I see different expression level (higher or lower) compared with my last result. I have no problem with the other 5 samples, but only this one.

What is the problem? Why this cell line's protein expression is not consistent with its last lysate?

p.s. I do not use any treatment. The origin of these cells are the same (colorectal). the passage and confluency is the same. the antibodies are the same, too.

Plz help me..................................

#2 EvilTwin

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Posted 03 January 2012 - 03:08 AM

Are these normal or cancer cells?

#3 cancerlab

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Posted 05 January 2012 - 05:01 PM

Are these normal or cancer cells?

Thanks for your reply. They aye all cancer cells.

#4 EvilTwin

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Posted 09 January 2012 - 12:03 AM

I think it might be the cause. Cancer cell are genetically unstable and some of their features may change division-to-division.

Edited by EvilTwin, 09 January 2012 - 01:17 AM.






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