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Western Blot showing band lower than anticipated (Mao-A)

Western Mao-A degradation phosphorylation

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#1 uqngrov2



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Posted 10 November 2011 - 11:04 PM

Hi All,
I am running a western blot from adult mice brain tissue (around 150 mg). Samples have been lysed as per a standard protocol (with protease inhibitors) that works on smaller brain tissue samples from rats, both adult and neonate. My protein of interest is Mao-A which is suppose to sit at 61 kDa on gel/membrane. In neonate rat, I get a single band at 61 kDa, in small samples of adult rat i get mostly 61 kDa and a smaller band around 50 kDa. In adult mice brain samples I am getting a faint band at 61 kDa and bright band at 50 kDa. Run different samples on same gel, using same lysis buffer to get these results as well as on different gels. I can't find anything in the literature re Mao-A at 50 kDa or why it is running 10 kDa lower than it should. I have thought of degradation or phosphorylation as cause but cannot find anything in literature to suggest this either. Anyone know what could be the problem??

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