1. Activate Jurkat T cells with 50 ng/ml PMA for 20h
2. replace media
3. infect cells with virus
4. replace media after 1h adherence time
5. incubate for 2d
6. protein prep
I wonder how long the Jurkat cells are activated for upon PMA?
Whether it will last for 2 days after first media replacement (step 2)?
Would I need to re-inject PMA after each media replacement to maintain the effects of PMA (i.e. maintain T cell activation) ?
Also, would this be toxic to the cell??
Thank you for your help.
Edited by jjj02027, 10 November 2011 - 12:25 AM.