Hi, I'm doing Bacillus Subtilis and I'm required to extract the total DNA from it. Is there any efficient extraction method for Bacillus?
I've try phenol chloroform method and the ethanol precipitation. But the amount of DNA extracted is too little that no band is spotted after I run a gel. Need help.....
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Legendary Graduate Beggar
Posted 15 November 2011 - 06:55 AM
For Gram +ve bacteria, it is better if you can crush/grind it with liquid nitrogen before you proceed with your PCI method.
What is your downstream application?
What is your downstream application?
Expecting the world to treat you fairly because you are a good person is like expecting the lion not to attack you because you are a vegetarian.
..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn’t kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434
..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn’t kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434
