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No band after extracting DNA from bacillus subtilis

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#1 babyoctopus



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Posted 09 November 2011 - 04:20 AM

Hi, I'm doing Bacillus Subtilis and I'm required to extract the total DNA from it. Is there any efficient extraction method for Bacillus?

I've try phenol chloroform method and the ethanol precipitation. But the amount of DNA extracted is too little that no band is spotted after I run a gel. Need help.....

#2 Adrian K

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Posted 15 November 2011 - 06:55 AM

For Gram +ve bacteria, it is better if you can crush/grind it with liquid nitrogen before you proceed with your PCI method.
What is your downstream application?
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..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...

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