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How to measure radioactive cholesterol (3H) in C eleganas?

c elegans cholesterol radioactivity

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#1 julianvr79



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Posted 07 November 2011 - 11:25 AM


a referee has asked to measure radioactive cholesterol uptake in C. elegans, does any have any idea of how to do this? Perkin Elmer sells cholesterol with tritium and 14C, I believe is better to use 3H , any ideas?? witch scintillation liquid is best with C. elegans or how many worms should I use per vial? do you think that ONE single worm will be measurable?
Thanks a lot

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