Is it possible to stably transfect mammalian cells using only a single vector? Or do you always need more than one vector?
1 reply to this topic
#1
Posted 06 November 2011 - 05:05 PM
#2
Posted 07 November 2011 - 08:14 AM
you will choose 2nd vector only in case your expression vector doesnt have the mammalian selection, otherwise only one vector is normally transfected for generating stable clones.
I would prefer being perfectionist rather than a passionist in Research.
I always had an alternate hypothesis....
I always had an alternate hypothesis....














