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stable transfection

transformation plasmid vector

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#1 JaimeeOlson

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Posted 06 November 2011 - 05:05 PM

Is it possible to stably transfect mammalian cells using only a single vector? Or do you always need more than one vector?

#2 GNANA

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Posted 07 November 2011 - 08:14 AM

you will choose 2nd vector only in case your expression vector doesnt have the mammalian selection, otherwise only one vector is normally transfected for generating stable clones.
I would prefer being perfectionist rather than a passionist in Research.

I always had an alternate hypothesis....





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