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Real-time mRNA expression studies on FFPE samples


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#1 Trof

Trof

    Brain on a stick

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Posted 05 November 2011 - 07:55 AM

Anyone did a quantification of mRNA on FFPE samples?

I will try two kits for total RNA isolation, one from QIAGEN (RNeasy FFPE) and some other, have anyone experience with the QIAGEN kit?
Do you know much is the yield of total RNA from recommended amount (2-4 slides thick up 20um) (I know it's pretty degraded)?
Are there problems with PCR inhibition?
Roughly what amount of total RNA you need to get reasonable Cts for housekeeping genes like GAPDH?
Any other important things about qPCR or RNA isolation of FFPE?

Sorry for the splash of questions, but I'm just starting with these Posted Image
Thanks for any answer.
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