Ligation needed after SDM with PfuX?
Posted 04 November 2011 - 01:22 PM
I am trying to make a mutation in my plasmid (9.5 Kbp) using long complementary primers (PAGE purified) and PCR. I am using Invitrogen's PfuX. Now, is my PCR product going to be linear? If yes, do I have to do a ligation after PCR? Or it has to be after each amplification cycle?
I noticed there is no ligation step in Strategen manual, is that a different procedure?
Posted 09 November 2011 - 12:13 AM
Posted 20 November 2011 - 08:20 AM
Posted 20 November 2011 - 08:35 AM
thanks for the explanation.