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How to stably integrate GFP protein into a mammalian cell line?

GFP stable transfection

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#1 pcong

pcong

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Posted 04 November 2011 - 07:45 AM

Hi,
I wanted to integrate GFP into a mammalian cell line.
First of all , which vector stable tranfection. Second, what are the step by step procedure for this experiment starting from choosing the vector, any gene manipulation, cloning ,maybe specific cell line , transfection process?
can anyone please fill me in with the basic detail and facts how i am going to proceed with it?

thanks
pcong





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