Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Bacterial expression: incorporating radioactivity into overexpressed protein

Bacterial expression radioactivity incorporating

  • Please log in to reply
1 reply to this topic

#1 TheBalrog



  • Active Members
  • Pip
  • 5 posts

Posted 04 November 2011 - 07:43 AM

I'd like to incorporate radioactive amino acids into a protein that's overexpressed in bacteria upon addition of IPTG. It doesn't really matter to me whether the hot amino acids are 35-S or 14-C, the most important thing is that the protein is as hot as possible so that it can be detected readily by autoradiography. So the questions:

1) Can anybody point me in the direction of some type of dropout medium that can be supplemented with hot amino acids?

2) Has anybody done such an experiment and found a high degree of radiolabel incorporation?

3) Does anybody have a reference to a paper that achieved the above?

Many thanks in advance.

#2 pDNA



  • Awaiting Authorisation
  • PipPipPipPipPipPipPipPipPipPip
  • 494 posts

Posted 05 November 2011 - 04:44 AM

there is a system available from takara, especially for protein labeling! ...i would go for such a system because otherwise your precious labled aminoacids are wasted on all the other E. coli proteins!

You can find the details [url="http://catalog.takara-bio.co.jp/en/product/basic_info.asp?unitid=U100005914"]here[/url]!

Good luck!

Best regards,

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.