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Can proteins with sizes smaller than 60 kda be seen as bands in a 6% gel western


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#1 labrat640

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Posted 04 November 2011 - 03:40 AM

I have a western blot for a 160 kda protein which was carried out using a 6% gel. Now I need to control for loading. I tried tubulin and gapdh but neither bands are present.

Any reasons why?

Are there other controls I can use?

Thanks,

#2 mdfenko

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Posted 04 November 2011 - 06:48 AM

they may have run off the gel.

can you run a gradient gel?
talent does what it can
genius does what it must
i do what i get paid to do

#3 cupidstunt

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Posted 10 November 2011 - 03:34 AM

try 8% gel. My proteins are 140 and 160 kDa and it works fine. I usually get beta actin at 8%.

#4 labrat640

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Posted 10 November 2011 - 07:18 AM

Thanks for your replies,

I have managed by repeating the western blot using 7.5 % gel and a loading control with alpha tubulin. Both WRN (160 kda) and alpha tubulin (70kda) bands are detected.




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