I have a western blot for a 160 kda protein which was carried out using a 6% gel. Now I need to control for loading. I tried tubulin and gapdh but neither bands are present.
Any reasons why?
Are there other controls I can use?
Thanks,
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Can proteins with sizes smaller than 60 kda be seen as bands in a 6% gel western
Started by labrat640, Nov 04 2011 03:40 AM
3 replies to this topic
#2
mdfenko
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Posted 04 November 2011 - 06:48 AM
they may have run off the gel.
can you run a gradient gel?
can you run a gradient gel?
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
#3
cupidstunt
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Posted 10 November 2011 - 03:34 AM
try 8% gel. My proteins are 140 and 160 kDa and it works fine. I usually get beta actin at 8%.
#4
labrat640
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Posted 10 November 2011 - 07:18 AM
Thanks for your replies,
I have managed by repeating the western blot using 7.5 % gel and a loading control with alpha tubulin. Both WRN (160 kda) and alpha tubulin (70kda) bands are detected.
I have managed by repeating the western blot using 7.5 % gel and a loading control with alpha tubulin. Both WRN (160 kda) and alpha tubulin (70kda) bands are detected.
