Can proteins with sizes smaller than 60 kda be seen as bands in a 6% gel western
Posted 04 November 2011 - 03:40 AM
Any reasons why?
Are there other controls I can use?
Posted 04 November 2011 - 06:48 AM
can you run a gradient gel?
genius does what it must
i do what i get paid to do
Posted 10 November 2011 - 03:34 AM
Posted 10 November 2011 - 07:18 AM
I have managed by repeating the western blot using 7.5 % gel and a loading control with alpha tubulin. Both WRN (160 kda) and alpha tubulin (70kda) bands are detected.