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Alternative to Sybr-green?


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12 replies to this topic

#1 stelane

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Posted 03 November 2011 - 01:36 PM

Hi,

Does anyone have any advice/experience/recommendations for an alternative to Sybr-green master mix? I'm getting a little annoyed with the price that SAB Biosciences are charging and was wondering if other people/labs had found cheaper alternatives for either sybr-green or at least suppliers of sybr-green?
Cheers,
Steve

#2 Archie

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Posted 04 November 2011 - 05:06 AM

Well, how much do you pay for your master mix?
Maybe it's worth trying to order bulk amounts together with other departments to get a higher discount?

#3 KevinK

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Posted 04 November 2011 - 08:00 AM

Promega recently launched qPCR mastermixes with a new dye we developed. Note that we list the various sizes by the number of reactions that can be performed with the product and we use a 50ul reaction size.

Hope this his useful.

Kevin
Promega Corporation
Madison, WI

#4 doxorubicin

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Posted 08 November 2011 - 04:20 PM

I've had great luck with the Roche master mix, and it is the least expensive one we can buy here. It is also possible to make your own master mix for a significant savings.

#5 Adrian K

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Posted 15 November 2011 - 06:51 AM

I've had great luck with the Roche master mix, and it is the least expensive one we can buy here. It is also possible to make your own master mix for a significant savings.


Just curious, how much you purchased?
Expecting the world to treat you fairly because you are a good person is like expecting the lion not to attack you because you are a vegetarian.

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#6 doxorubicin

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Posted 15 November 2011 - 09:17 AM

I'm in Europe and our Institut has a "store" with certain items that they keep in stock. It costs us 110 euros for 5mL. If you want the recipe to make your own master mix email me: doxorubicin2001@yahoo.com

#7 Neanderthal

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Posted 16 November 2011 - 12:20 AM

Hi,

Does anyone have any advice/experience/recommendations for an alternative to Sybr-green master mix? I'm getting a little annoyed with the price that SAB Biosciences are charging and was wondering if other people/labs had found cheaper alternatives for either sybr-green or at least suppliers of sybr-green?
Cheers,
Steve


Have you checked SsoFast™ EvaGreen® Supermix from Bio-Rad?

#8 leelee

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Posted 16 November 2011 - 01:40 AM

I've had great luck with the Roche master mix, and it is the least expensive one we can buy here.


We also use the Roche master mix.

#9 stelane

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Posted 16 November 2011 - 08:12 AM

Thanks for the replies, I'll look into the bio-rad product. I have spoken to Roche about their mastermix, but unfortunately they are not willing to supply a small sample to validate against our system and previous results. Since I already have a bunch of results using the SABiosciences product I'm not willing to pay more money for a different product only to find it introduces more variability to those results. Once I've finished my current work though I think I'll try Roche.

#10 doxorubicin

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Posted 16 November 2011 - 10:44 AM

Here they have a small sample you can purchase for 1 euro.......

#11 Adrian K

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Posted 17 November 2011 - 06:29 AM


I've had great luck with the Roche master mix, and it is the least expensive one we can buy here.


We also use the Roche master mix.


How much is the price in Australia?
Expecting the world to treat you fairly because you are a good person is like expecting the lion not to attack you because you are a vegetarian.

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#12 Trof

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Posted 18 November 2011 - 12:02 AM

Roche mix works best for our system (but you should know we have Roche machine and RT enzyme) but I wouldn't call it least expensive, it's just the oposite here as I recall (490 EUR for 5ml = 1000 reactions). But we tested this and ABI mixes and Roche mix had slightly lower Cts. Then we tested something from Holland that costed half the price and it didn't show any results at all for some strange reason and the person from that company contacted me repeatedly and wanted to test what went wrong, but I wasn't interested in trying it more, annoying guy.

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#13 leelee

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Posted 20 November 2011 - 06:53 PM



I've had great luck with the Roche master mix, and it is the least expensive one we can buy here.


We also use the Roche master mix.


How much is the price in Australia?


Pretty pricey actually- but then I haven't looked at the prices of other products. We have the Roche LC480 machine and all of our assays have been worked up using the Roche master mix (probes master) so I'd be hesitant to have to redo all that validation with a new product. (plus Roche have been pretty good with their tech support to date).

We are paying around $4000 AU (which is about $4000 US, and about $2900 Euros) for 10x5ml of the probes master (and usually get a case of 50x 96 well plates for free with that).




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