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Glycerol stocks of anaerobic bacteria

microbiology

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#1 toby_samuels

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Posted 02 November 2011 - 04:38 AM

Hi

Ok so I have cultured from freeze dried stocks Bifidbacterium bifidium and Colinsella aerofaciens anaerobic conditions in liquid culture and streaked onto a plate.

I am wondering if I can simply make them up into glycerol stocks as normal or do I need to do anything extra?

May sound like a simple question, but not worked with anaerobes before and don't want to lose my cultures!

I realise I also need to verify that I have cultured the desired bacteria and not contaminants - thats my next step!

Any help would be greatly appreciated!

T

#2 pito

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Posted 02 November 2011 - 11:11 AM

Not ure if I understand what you mean.
But yes, you can store then in glycerol.
You just need to be carefull and ensure you work anaerobicly.
Check out https://wiki.science...igate_anaerobes for more information.

If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.






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