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PLATINUM TAQ FOR MSP?

PLATINUM TAQ MSP

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2 replies to this topic

#1 genie

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Posted 02 November 2011 - 04:08 AM

Hi Everybody,

Planning to do MSP-PCR on bisulfite treated DNA. I ordered the Platinum Taq for the MSP-PCR.
I later read in this forum that a proofreading polymerase (i.e polymerase with 3'-5' mismatch repair) can not be used in MSP.

Does anyone know if Platinum Taq would qualify for use in MSP? i did find a few references that did use it though.
but if someone recalls that this combination did'nt work for them, then are there any better suggestions for Taq used in MSP-PCR?

hope to get some advice on this matter.

Thanks for any help.

Edited by genie, 02 November 2011 - 04:16 AM.


#2 methylnick

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Posted 04 November 2011 - 03:32 AM

you may not get efficient amplification with proof reading Taq and Platinum is a proofreading one, I would switch to a hot start taq. It's cheaper too.

N

#3 genie

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Posted 04 November 2011 - 06:19 AM

Thanks Nick for your reply. will look for a hotstart taq in the lab.




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