Protein of 160 kda is present on 6% gel but gapdh housekeeping protein is not.
Posted 02 November 2011 - 02:51 AM
Bands for either GAPDH (approximately 40 kda) or tubulin (50kda) were absent after stripping the membrane and reprobing each time.
Is it because the protein sizes are too small for a 6% gel? A ladder is loaded and demonstrates different sizes on the gel (from 250 kda to 10 kda).
What loading control can I use?
Posted 02 November 2011 - 08:09 AM
Posted 02 November 2011 - 11:21 AM
I have just seen a list of recommended gel concentrations, it suggests a 6% gel for 60-300 kda.
What control can I use for loading?
Posted 02 November 2011 - 06:21 PM
Posted 03 November 2011 - 08:42 AM
The question is: can protein bands the size of GapDH and Actin be visible on a 6 % gel or not?
Posted 04 November 2011 - 09:07 AM
Are there any loading controls larger than 60 kda?
I think the solution is to run a 7.5% gel. I will give it a try.