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a few questions about ion exchange chromatography

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31 replies to this topic

#31 xaxax



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Posted 19 January 2012 - 04:31 AM

if i use affinity purification, after this step, i must reduce the salt and buffer concentration when i want to make ion exchange.
Should I use desalting columns for this?
Maybe, after affinity, gel filtration is enough.
I will post my results whenever I get.

#32 mdfenko


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Posted 19 January 2012 - 08:03 AM

you can use desalting (gel filtration) columns or you can dialyze.

be careful, with reduced ionic strength there is more chance of non-specific binding to the matrix.

talent does what it can
genius does what it must
i used to do what i got paid to do

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