if i use affinity purification, after this step, i must reduce the salt and buffer concentration when i want to make ion exchange.
Should I use desalting columns for this?
Maybe, after affinity, gel filtration is enough.
I will post my results whenever I get.
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mdfenko
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Excellent
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Posted 19 January 2012 - 08:03 AM
you can use desalting (gel filtration) columns or you can dialyze.
be careful, with reduced ionic strength there is more chance of non-specific binding to the matrix.
be careful, with reduced ionic strength there is more chance of non-specific binding to the matrix.
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