a few questions about ion exchange chromatography
Posted 19 January 2012 - 04:31 AM
Should I use desalting columns for this?
Maybe, after affinity, gel filtration is enough.
I will post my results whenever I get.
Posted 19 January 2012 - 08:03 AM
be careful, with reduced ionic strength there is more chance of non-specific binding to the matrix.
talent does what it can
genius does what it must
i used to do what i got paid to do