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Non specific binding of radioligands question


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#1 john909

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Posted 01 November 2011 - 04:00 PM


I have a quick question. I'm having difficulty understanding why non specificity in ligand binding experiments is desired to be kept to a minimum. What are the disadvantages of having an experiment which results in high non specific radioligand binding?

thanks for your time

John





#2 leelee

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Posted 01 November 2011 - 07:13 PM

Ok well think about it, if you are using a radioligand to measure/detect/visualise a specific receptor- what use is it if it binds to something other than what you are interested in?

#3 mdfenko

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Posted 03 November 2011 - 05:51 AM

think "background" and signal to noise ratio
talent does what it can
genius does what it must
i do what i get paid to do




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