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protocol for making e.coli competent cells under low temperature

Started by nainiburman21, Nov 01 2011 05:58 AM

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#1 nainiburman21

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Posted 01 November 2011 - 05:58 AM

Hi, I have heard that e.coli chemical competent cells made under low temperature i.e below 37C are very competetive. Does any one has protocol for it?

#2 bob1

    Hmmm, I think it's working

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Posted 02 November 2011 - 04:20 PM

Here you go:http://openwetware.o...competent_cells
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