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self cleavage of GST from the HIS-SUMO-GST fusion protein

sumo gst

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#1 belle713



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Posted 01 November 2011 - 04:31 AM


I'm purifying HIS-SUMO-GST protein, the expression is done under 37C for 4.5 hr and the protein is purified using Nickel column followed by gel filtration. I want to keep the GST tag with the SUMO, but then there is self cleavage of GST.

any ideas for preventing the GST from falling off itself?

thanks a million !

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