Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Help with reverse adaptation

Cell Culture

  • Please log in to reply
No replies to this topic

#1 briguy7

briguy7

    member

  • Active Members
  • Pip
  • 24 posts
1
Neutral

Posted 31 October 2011 - 10:18 AM

I have a couple of suspension cell lines that I am thinking about using for developing a binding assay--I wanted to run it sort of like an ELISA with my cells stuck to the plate and after adding my protein I will wash and add conjugated reporter antibody. The problem I am having is I can't find any adherent cell lines that work--I am focusing on HER2 or CD20 lines and they would all be bone marrow derived cells. Is there any way I can adapt a cell line to grow adherently? If not, is there a coating I can put on my plate that will induce them to bind to the plate strong enough to allow me to wash unbound protein from my plate before I add my reporter antibody?





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.