I have been using 5-azacytidine on cancer cell lines. I have noticed strong reactivation at the protein level of my gene of interest when I apply 5-azaC. However, I have ntoiced that after sodium bisulphite sequencing the CpG island within my gene of interest is still methylated. I was wondering if there was a positve control that is frequently used to assess the efficiency of the 5-azaC treatment.
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