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5-azacytidine treatment


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#1 Louie

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Posted 31 October 2011 - 08:12 AM

Hi

I have been using 5-azacytidine on cancer cell lines. I have noticed strong reactivation at the protein level of my gene of interest when I apply 5-azaC. However, I have ntoiced that after sodium bisulphite sequencing the CpG island within my gene of interest is still methylated. I was wondering if there was a positve control that is frequently used to assess the efficiency of the 5-azaC treatment.

Thanks

#2 methylnick

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Posted 04 November 2011 - 03:30 AM

I don't think there is a control to test 5-aza-C efficiency, it would be cell line specific also.




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