I have a enzyme and I know its Km, Vm and Kcat value but I dont know its Kd value. How to drive the Kd value from Km value. I shall be very thankful to you for your kind reply.
best regards
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3 replies to this topic
#2
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Posted 02 November 2011 - 07:09 AM
The Km is approximately the KD (dissociation constant) for the substrate
#3
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Posted 16 February 2012 - 01:29 PM
Agree with Bluefunk. Km and Kd are different , but at the same order. Usually, they are measured with different assays. I do not think you can convert Kd from Km.
#4
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Posted 22 February 2012 - 06:25 PM
dissociation constant(Kd) is a specific type of equilibrium constant that measures the propensity of a larger object to separate (dissociate) reversibly into smaller components, as when a complex falls apart into its component molecules, or when a salt splits up into its component ions.
