Posted 31 October 2011 - 01:32 AM
RNAi involves the cleavage of dsRNA into small fragments and the recruitment of RISC; while
Antisense RNA is the mere inhibition of translation by complementary pairing?
If that's correct, wouldn't antisense RNA always lead to RNAi?
Posted 31 October 2011 - 06:50 AM
RNAi is the alteration of gene expression involving the RISC system. Since neither RNase-H competent antisense nor steric-blocking antisense involves RISC activity, these are not RNAi. However, they do alter gene expression though their different pathways.
This paper discusses these kinds of antisense & siRNA: www.benthamscience.com/ctmc/openaccessarticles/ctmc7-7/0003R.pdf
Edited by Jon Moulton, 31 October 2011 - 12:36 PM.
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