Comparison between two primers pairs? Is it possible?
Posted 29 October 2011 - 11:12 AM
The problem is.. This region has many repetitions.. And the primers I designed for one strain are perfect for that strain, BUT I had to design other primer pair for the other strain!
Amplicons have almost the same size (difference of 1bp) but the GC content of the F primer of one pair is around 20% different from the other one and the R primers have almost the same GC content..
My question is.. do you think I could compare the relative expression of this gene (already normalized), using the mentioned TWO PRIMER PAIRS between the strains using the delta CT method? Or i should run any efficiency test..?
Posted 29 October 2011 - 04:57 PM
Posted 29 October 2011 - 07:39 PM