pH change after ATP addition
Posted 29 October 2011 - 09:49 AM
Posted 29 October 2011 - 01:42 PM
If you are dissolving all three together at the same time, you should probably try dissolving the ATP in the buffer after you have made it.
If you are dissolving the ATP in pre-made buffer, then you could try adding more phosphates, but you would alter the concentration of the salts which would alter the osmolarity. I think you would be better off pHing with NaOH.
Posted 31 October 2011 - 04:04 AM
Posted 31 October 2011 - 08:13 AM
you can also pre-adjust the atp then add the concentrated stock phosphate buffer and bring to final volume.
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