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Albumin contamination


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#1 njeffre

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Posted 28 October 2011 - 12:41 PM

I have produced whole immunoglobilins but have albumin in my sample. What is the best way to remove the albumin from my sample. I do not have chromatography as an option.

#2 BioMiha

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Posted 28 October 2011 - 11:30 PM

GE Healthcare (and probably others as well) have albumin depleting kits for 2D PAGE. Maybe you could give it a try. Depends on the amount of sample and albumin you have.

#3 njeffre

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Posted 31 October 2011 - 10:43 AM

This will be scaled up to a production scale project so I need to be able to scale up the albumin depletion as well. Thanks for the suggestion though. I will try to see what they use and try to come up with something similar that can be scaled up.




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