I have produced whole immunoglobilins but have albumin in my sample. What is the best way to remove the albumin from my sample. I do not have chromatography as an option.
Submit your paper to J Biol Methods today!
2 replies to this topic
#2
BioMiha
-
- Active Members
-
- 237 posts
Veteran
11
Good
Good
Posted 28 October 2011 - 11:30 PM
GE Healthcare (and probably others as well) have albumin depleting kits for 2D PAGE. Maybe you could give it a try. Depends on the amount of sample and albumin you have.
#3
njeffre
-
- Members
-
- 4 posts
member
0
Neutral
Neutral
Posted 31 October 2011 - 10:43 AM
This will be scaled up to a production scale project so I need to be able to scale up the albumin depletion as well. Thanks for the suggestion though. I will try to see what they use and try to come up with something similar that can be scaled up.
