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ROS measurement

ROS GFP-tagged cells DCF Luminol assay

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#1 Thapa

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Posted 28 October 2011 - 09:07 AM

Hi,
Because my cells are GFP-labelled, I am looking for the best alternative to DCF-DA assay. I have just seen a paper about the "luminol-dependent chemiluminescence assay" for ROS measurement. I dont know whether it works in GFP-labelled cells or I wonder if theres any other methods to quantify ROS production in my cells....???

Thanks a lot.

Thapa
"Learning without thought is labor lost"

#2 zienpiggie

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Posted 28 October 2011 - 09:36 AM

There are a number of different probes that you can use depending on the type of free radicals you are targeting. Here's a list I found from invitrogen website http://www.invitroge...en-species.html .

#3 Thapa

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Posted 28 October 2011 - 11:44 AM

Thanks a lot Zienpiggie for the package of information; the web link seems worthy for me.

By the way, it would be nice if one of us have done expts in a similar settings (using GFP labelled cells) and would like to share the experience.
"Learning without thought is labor lost"





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