Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

SENP inhibitor (SUMO)

SUMO SENP immunoprecipitation

  • Please log in to reply
No replies to this topic

#1 jclee

jclee

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 26 October 2011 - 08:13 PM

Hi,

I am interested in SUMOylation of some target proteins, and tried to study it by immunoprecipitation of the protein of interest from cell lysate. However, the SUMOylated status is so labile, I could not get convincing result. Some researchers used NEM (N-ethylmaleimide) as an inhibitor of SUMO proteases, but it is very toxic so I hesitate to use it. Has anyone tried specific SENP inhibitors, such as SUMO aldehyde and SUMO vinyl sulfone by ENZO or BostonBiochem or else? How do they work? Do I just add them into the cell lysis buffer before use? Many thanks!


J





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.