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[jclee]

Hi,

I am interested in SUMOylation of some target proteins, and tried to study it by immunoprecipitation of the protein of interest from cell lysate. However, the SUMOylated status is so labile, I could not get convincing result. Some researchers used NEM (N-ethylmaleimide) as an inhibitor of SUMO proteases, but it is very toxic so I hesitate to use it. Has anyone tried specific SENP inhibitors, such as SUMO aldehyde and SUMO vinyl sulfone by ENZO or BostonBiochem or else? How do they work? Do I just add them into the cell lysis buffer before use? Many thanks!


J