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Why is my Cre PCR data does not match the immunofluorescent-labeling?

Cre conditional knockout mice

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#1 clc

clc

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Posted 26 October 2011 - 03:13 PM

Hi,

Have you experienced this: you genotyped a mouse (ear clip) and found it to be Cre-positive; then you take the tissue and do immunostaining but there's no labeling with the Cre antibody!

I have a positive control making sure the Cre antibody and my method works.

Any thoughts?

Thanks!





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