My question is if I can reblot a membrane with ghost bands again with the same antibody but less concentrate. Is this possible or I have to repeat the gel?
Thanks
Irene
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2 replies to this topic
#1
Irene G
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Posted 26 October 2011 - 02:40 AM
Hi!
My question is if I can reblot a membrane with ghost bands again with the same antibody but less concentrate. Is this possible or I have to repeat the gel?
Thanks
Irene
My question is if I can reblot a membrane with ghost bands again with the same antibody but less concentrate. Is this possible or I have to repeat the gel?
Thanks
Irene
#2
BioMiha
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Posted 26 October 2011 - 05:18 AM
The Abs don't wash away. You would have to strip the membrane and then reprobe, but this is not very reproducible.
#3
Irene G
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Posted 26 October 2011 - 05:42 AM
Thank you, I will make a new gel!
