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Re-using membranes with ghost bands

Ghost bands

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2 replies to this topic

#1 Irene G

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Posted 26 October 2011 - 02:40 AM

Hi!
My question is if I can reblot a membrane with ghost bands again with the same antibody but less concentrate. Is this possible or I have to repeat the gel?
Thanks
Irene

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#2 BioMiha

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Posted 26 October 2011 - 05:18 AM

The Abs don't wash away. You would have to strip the membrane and then reprobe, but this is not very reproducible.

#3 Irene G

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Posted 26 October 2011 - 05:42 AM

Thank you, I will make a new gel!




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