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ChIP from mouse tissues with antibody raised in mouse

mouse tissue ChIP

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#1 KDK

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Posted 25 October 2011 - 08:46 PM

Is there any reason why you cannot ChIP from mouse tissue with an antibody raised in mouse?

#2 chabraha

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Posted 01 November 2011 - 10:19 AM

Ideally no, technically perhaps. I think you should just do the ChIP and find out........I'd be interested in the results. The only problems I for-see is 1) mouse monoclonals aren't the best for ChIPs 2) Fc Receptors from resident immune cells could function as a molecular sink, binding up some of your antibody........but that probably depends on your tissue......and your isotype control should help you see if that were the case.
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