Selection of cell ine
Posted 23 February 2003 - 10:05 PM
Posted 23 February 2003 - 10:09 PM
Posted 27 February 2003 - 09:11 AM
(2) Set up a in vitro and/or cell based assay using recombinant protein or whole cells.
(3) select your functional readout for detection e.g. fluorescence in kinase, caspase...or cell death index for apoptosis- MTT, JC-1 dye....
(4) Screen the compound library for inhibiting target kinase or activating caspase or killing cells.