Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

Condition for Transformation - heat shock, "A novice"

broth time tubes

  • Please log in to reply
2 replies to this topic

#1 CAT

CAT

    member

  • Active Members
  • Pip
  • 24 posts
0
Neutral

Posted 25 October 2011 - 11:35 AM

Hi,

I am confused at reading different protocols for heat shock. Anyone please give a clue. Thanks! Posted Image

For the same strain (say XL-blue), I have found:


1. one writes NZY+ broth, while another one writes SOC.

2. And for the time at 42C, One said 45 seconds, the other said 30 seconds.

3. Then for the tubes, falcon 2059 polypropylene tube, VS eppendorf vial

Edited by CAT, 25 October 2011 - 11:36 AM.


#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,598 posts
384
Excellent

Posted 25 October 2011 - 06:22 PM

These methods are all based on work done by Douglas Hanahan published in 1983. You should read his paper to find out why...

In general most of these sorts of things don't matter too much, but can sometimes lower the transformation efficiency drastically. The exact conditions you use should be optimised based on what you are using!
  • CAT likes this

#3 CAT

CAT

    member

  • Active Members
  • Pip
  • 24 posts
0
Neutral

Posted 26 October 2011 - 05:51 AM

Thanks, bob1. This paper helps a lot. Great!Posted Image





Also tagged with one or more of these keywords: broth, time, tubes

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.