I am confused at reading different protocols for heat shock. Anyone please give a clue. Thanks!
For the same strain (say XL-blue), I have found:
1. one writes NZY+ broth, while another one writes SOC.
2. And for the time at 42C, One said 45 seconds, the other said 30 seconds.
3. Then for the tubes, falcon 2059 polypropylene tube, VS eppendorf vial
Edited by CAT, 25 October 2011 - 11:36 AM.