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Canine raw plasma

serology antibodies canine albumin

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2 replies to this topic

#1 njeffre

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Posted 25 October 2011 - 11:19 AM

I have been working for the past few months on developing a procedure for producing albumin and immunoglobulins from raw plasma. Can anyone give me a non-chromatographic method for precipitation of IgG, IgM, and IgA? If possible the supernatant needs to be useable in order to concentrate the albumin remaining in the fraction.

#2 Mohamad Zulkeflee Sabri

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Posted 19 December 2011 - 05:34 PM

you can try immunoprecipitation method using anti-IgG or protein A. The protocol is simpler but I am not sure how good the yield is.

#3 PAO_ahac

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Posted 21 December 2011 - 06:01 AM

SAS...saturated ammonium sulfate precipitation will bring down your IgGs with some contamination of transferrin and albumin. You then need to separate out the specific classes





Also tagged with one or more of these keywords: serology, antibodies, canine, albumin

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