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Canine raw plasma

serology antibodies canine albumin

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2 replies to this topic

#1 njeffre

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Posted 25 October 2011 - 11:19 AM

I have been working for the past few months on developing a procedure for producing albumin and immunoglobulins from raw plasma. Can anyone give me a non-chromatographic method for precipitation of IgG, IgM, and IgA? If possible the supernatant needs to be useable in order to concentrate the albumin remaining in the fraction.

#2 Mohamad Zulkeflee Sabri

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Posted 19 December 2011 - 05:34 PM

you can try immunoprecipitation method using anti-IgG or protein A. The protocol is simpler but I am not sure how good the yield is.

#3 PAO_ahac

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Posted 21 December 2011 - 06:01 AM

SAS...saturated ammonium sulfate precipitation will bring down your IgGs with some contamination of transferrin and albumin. You then need to separate out the specific classes





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