I have been working for the past few months on developing a procedure for producing albumin and immunoglobulins from raw plasma. Can anyone give me a non-chromatographic method for precipitation of IgG, IgM, and IgA? If possible the supernatant needs to be useable in order to concentrate the albumin remaining in the fraction.
0
2 replies to this topic
#2
Mohamad Zulkeflee Sabri
-
- Active Members
-
- 6 posts
member
0
Neutral
Neutral
Posted 19 December 2011 - 05:34 PM
you can try immunoprecipitation method using anti-IgG or protein A. The protocol is simpler but I am not sure how good the yield is.
#3
PAO_ahac
-
- Active Members
-
- 94 posts
Enthusiast
0
Neutral
Neutral
Posted 21 December 2011 - 06:01 AM
SAS...saturated ammonium sulfate precipitation will bring down your IgGs with some contamination of transferrin and albumin. You then need to separate out the specific classes
Also tagged with one or more of these keywords: serology, antibodies, canine, albumin
Protocols and Techniques Forums →
Immunology →
Serum inactivation:Started by Guest_@bhijit_* , 20 Nov 2012  serum, antibodies
|
|
|
||
Protocols and Techniques Forums →
SDS-PAGE and Western Blotting →
HELP!! Anti-GFP antibody resuspended in 20% SDS!!!!Started by Guest_SPombe3_* , 24 Oct 2012 antibody, antibodies, SDS and 2 more...
|
|
|
||
Protocols and Techniques Forums →
Molecular Cloning →
about a bookStarted by Guest_majidatf_* , 12 Oct 2012 antibodies
|
|
|
||
Protocols and Techniques Forums →
Histology and Pathology →
Problem with sequential double IF staining with rabbit antibodiesStarted by Guest_alexkoon_* , 27 Sep 2012 IF, antibodies, immunostaining and 2 more...
|
|
|
||
Protocols and Techniques Forums →
ChIP and Next Generation Sequencing →
Ab for acetyl modifications in low-input ChIP experimentsStarted by Guest_emilyindallas_* , 25 Sep 2012 Antibodies, low-input, ChIP
|
|
|
