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primary cell isolation from CNS, how to remove myeline debris

myelin debris

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#1 Rnotk

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Posted 25 October 2011 - 10:21 AM

Hey guys

I have a question for you,
I just started to do some culture of primary cell from mouse CNS,
but after dissociation/isolation, myeline debris is all over and make it almost impossible for me to count the # of cells.

Does anyone know some easy (and hopefully inexpensive way) to remove the debris?

I know there is myeline removal microbeads in market, but I prefer some other method since our lab does not use the microbeads system.

I prefer some density gradient protocol, so if anyone have any suggestion, please let me know.

Thanks in advance

#2 LittleMissTcell

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Posted 12 January 2012 - 06:53 AM

Myelin debris is a pain for counting! What cells are you culturing and how old are the mice?




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