1 reply to this topic

[Rnotk]

Hey guys

I have a question for you,
I just started to do some culture of primary cell from mouse CNS,
but after dissociation/isolation, myeline debris is all over and make it almost impossible for me to count the # of cells.

Does anyone know some easy (and hopefully inexpensive way) to remove the debris?

I know there is myeline removal microbeads in market, but I prefer some other method since our lab does not use the microbeads system.

I prefer some density gradient protocol, so if anyone have any suggestion, please let me know.  

Thanks in advance

[LittleMissTcell]

Myelin debris is a pain for counting!  What cells are you culturing and how old are the mice?