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cell culture media refrigeration


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#1 gnarl

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Posted 24 October 2011 - 11:31 PM

If DMEM a) with L-glutamine B) without L-glutamine is not refrigerated for 1-2 days, what effect will this have?

Thanks!

#2 leelee

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Posted 24 October 2011 - 11:40 PM

Homework question?

#3 rhombus

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Posted 25 October 2011 - 04:42 AM

It is not shipped refridgerated....does that answer your question?????????

Kindest regards.

Uncle Rhombus.

#4 gnarl

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Posted 26 October 2011 - 10:00 AM

thanks for the replies. is there any harm associated with degraded l-glutamine?

#5 leelee

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Posted 26 October 2011 - 06:37 PM

Our media with L-glutamine in it comes shipped cold.
That said- 1 or 2 days at room temp should be fine (if you think about it, your media will be at 37C for that amount of time before you replace it).

I usually replenish L-glu after a month in the fridge.

If this is an ongoing concern of yours, you could consider either purchasing media with a stable l-glu substitute, or purchase media l-glu free and then keep frozen aliquots of l-glu to add just prior to using the media?




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