without L-glutamine is not refrigerated for 1-2 days, what effect will this have?Thanks!
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#1
gnarl
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Posted 24 October 2011 - 11:31 PM
If DMEM a) with L-glutamine without L-glutamine is not refrigerated for 1-2 days, what effect will this have?
Thanks!
without L-glutamine is not refrigerated for 1-2 days, what effect will this have?Thanks!
#2
leelee
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Posted 24 October 2011 - 11:40 PM
Homework question?
#3
rhombus
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Posted 25 October 2011 - 04:42 AM
It is not shipped refridgerated....does that answer your question?????????
Kindest regards.
Uncle Rhombus.
Kindest regards.
Uncle Rhombus.
#4
gnarl
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Posted 26 October 2011 - 10:00 AM
thanks for the replies. is there any harm associated with degraded l-glutamine?
#5
leelee
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Posted 26 October 2011 - 06:37 PM
Our media with L-glutamine in it comes shipped cold.
That said- 1 or 2 days at room temp should be fine (if you think about it, your media will be at 37C for that amount of time before you replace it).
I usually replenish L-glu after a month in the fridge.
If this is an ongoing concern of yours, you could consider either purchasing media with a stable l-glu substitute, or purchase media l-glu free and then keep frozen aliquots of l-glu to add just prior to using the media?
That said- 1 or 2 days at room temp should be fine (if you think about it, your media will be at 37C for that amount of time before you replace it).
I usually replenish L-glu after a month in the fridge.
If this is an ongoing concern of yours, you could consider either purchasing media with a stable l-glu substitute, or purchase media l-glu free and then keep frozen aliquots of l-glu to add just prior to using the media?
