cell culture media refrigeration
Posted 24 October 2011 - 11:31 PM
Posted 25 October 2011 - 04:42 AM
Posted 26 October 2011 - 10:00 AM
Posted 26 October 2011 - 06:37 PM
That said- 1 or 2 days at room temp should be fine (if you think about it, your media will be at 37C for that amount of time before you replace it).
I usually replenish L-glu after a month in the fridge.
If this is an ongoing concern of yours, you could consider either purchasing media with a stable l-glu substitute, or purchase media l-glu free and then keep frozen aliquots of l-glu to add just prior to using the media?