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Binding of reduced antibody to Protein-A??


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#1 rick112

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Posted 24 October 2011 - 08:59 PM

Hi

I have recently observed that when antibodies (IgG1) are reduced they fail to bind to “Protein A” (from Staphylococcus aureus)?? why is this so, is the whole Fc part of the antibody needed for the binding with ‘Protein-A” ?? Doesn’t single chain “heavy-chain” bind to them??

#2 mdfenko

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Posted 25 October 2011 - 08:40 AM

protein a (and g) binds to the fc (tail) part of the antibody.
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