Is it necessary to add a short sequence between a restriction site and the promoter while cloning? Can the promoter sequence immediately follow the restriction site sequence?
0
2 replies to this topic
#2
pDNA
-
- Active Members
-
- 489 posts
Veteran
14
Good
Good
Posted 28 October 2011 - 10:00 AM
yes it can ...there should be no interference (not to my knowledge)!
Regards,
p
Regards,
p
Also tagged with one or more of these keywords: cloning, promoter
 |
Protocols and Techniques Forums →
Molecular Cloning →
"Easiest" sticky end combinationStarted by Guest_doxorubicin_* , Today, 03:45 AM  ligation, cloning and 1 more...
|
|
|
|
|
|
Protocols and Techniques Forums →
PCR, RT-PCR and Real-Time PCR →
Reproducible Non-Specific PCR ProductStarted by Guest_Epigeneticist_* , Yesterday, 04:56 PM PCR, Sequencing, Cloning
|
|
|
|
|
|
Protocols and Techniques Forums →
Molecular Cloning →
classic cloning with restriction enzymes into empty pcDNA3.1DStarted by Guest_Euterpa12_* , 17 May 2013 cloning, pcDNA3.1
|
|
|
|
|
|
Protocols and Techniques Forums →
Molecular Cloning →
Cloning large fragmentsStarted by Guest_bknm_* , 15 May 2013 Cloning, PCR, large inserts
|
|
|
|
|
|
Protocols and Techniques Forums →
Molecular Cloning →
Transformation colonies does not contain insertStarted by Guest_Sandy Fowler_* , 08 May 2013 transformation, Ligation, Cloning
|
|
|
