Is it necessary to add a short sequence between a restriction site and the promoter while cloning? Can the promoter sequence immediately follow the restriction site sequence?
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Also tagged with one or more of these keywords: cloning, promoter
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classic cloning with restriction enzymes into empty pcDNA3.1DStarted by Guest_Euterpa12_* , Yesterday, 08:13 AM |
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Molecular Cloning →
Cloning large fragmentsStarted by Guest_bknm_* , 15 May 2013 |
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Protocols and Techniques Forums →
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Transformation colonies does not contain insertStarted by Guest_Sandy Fowler_* , 08 May 2013 |
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Need urgent advise..Started by Guest_M.T2013_* , 29 Apr 2013 |
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Protocols and Techniques Forums →
Molecular Cloning →
difficulty to clone and/or express proteases. Toxicity?Started by Guest_monicalvi_* , 19 Apr 2013 |
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